Abstract: The hemp bundles were submerged in clear water and sea water. After 9 days, the water used for submerging was diluted to volume fraction 0.01% by gradient method, and then applied uniformly on the culture medium sheet and the dominant single colony was selected through cultivating, separating and purifying. Thus obtained respectively two strains named 1309 and 2508 that were more effective in decomposing the pectin of the hemp. Both of the strains are Gram-negative. Strain 1309 is a bacillus with polar flagella in motion, while strain 2508 is a round-ended bacillus with polar flagellum in motion. Through biological analysis, preliminary results reveal that strain 1309 is Pseudomonas spp., and 2508 is Stenotrophomnas maltophilia.