Abstract: In order to improve the enzymatic scouring effect,compound enzymes have received growing attention.However,the optimal scouring conditions vary with enzyme preparation,which has greatly limited the application of compound enzymes.Therefore,one-step one-bath scouring of knitted cotton fabrics with home_made alkaline pectinase,neutral cellulose,neutral-alkaline protease and xylanase as well as their combinations was investigated in the present work.The enzyme combinations for cotton scouring were optimized.The results showed that compound enzymes scouring exhibits better effect than individual enzymes,and wettability and dyeing properties that are very close to the conventional scouring but less strength loss.