Abstract: In view of wool fabric anti-bacterial finish, a new biological cross-linker MTG（transglutaminase）was used to catalyze the immobilization of lactoferrin onto the wool fabric and the antibacterial properties of the enzyme treated wool were tested. The lactoferrin immobilization efficiency was determined by UV spectrophotometry. Major parameters in immobilizing process were discussed. The optimum conditions were as follows: 30U/g wool fabric of MTG, 40℃ of temperature, 2h of reactive time, 5mg/mL of lactoferrin. Under these conditions, the lactoferrin immobilization efficiency on wool fabrics was about 9%, the inhibition rate to staphyllo-COCCUS aureus reached 57.95％ and the treated fabric exhibited good antibacterial property.