Abstract: In order to study the serviceability of the Nano-TiO2 antibacterial cotton fabric, FAST system is employed to measure the compression, bending, shearing, extension and other low stress mechanical properties of cotton fabrics with and without antibacterial finishing by Nano-TiO2 in this paper. The structural parameter and dimensional stability are also tested. The results show that weight, weft density, surface thickness, bending, formability, shearing and relaxation shrinkage of weft direction increased slightly, while shearing at warp 45°, relaxation shrinkage of warp direction and hygral expansion decreased. As a whole, most of the mechanical properties are still within the normal range and some is even better, its serviceability also remains stable.