Abstract: In order to prepare antibacterial cotton knitted fabric,In this paper,use nano-silicone acrylate emulsion whice contain silver ions to finish cotton knitting fabric,study on liquor ratio、pH、curing temperature and time which affect antibacterial effect of finfished cotton knitted fabric,use the rate of antibacterial as a measure of antibacterial effect,Obtained better antimicrobial finishing conditions by single-factor test: liquor ratio1︰50、pH=5、curing temperature 160℃、curing time 180s.And then whiteness、mechanical properties、permeability、antibacterial effect and washing durability of finfished cotton knitted fabric were tested，whiteness was down 20.6%, bursting strength keep at 81%,the rate of permeability has dropped 15%, the rate of antibacterial against S.aureus and E.coli is more than 98%, and the rate of antibacterial of antibacterial Fabric maintained above 80% after washed 30 times.