Abstract: This study use the Polyvinyl Alcohol to make the nanofiber mat by Non-syringe electrospinning process, we can find the result of different fiber’s diameters on different distance from spin roller to electrode plate. The average diameter is 165 nm (SD. is 49 nm) on 12 cm of the distance from spin roller to electrode plate, another; the average diameter is 219 nm (SD. is 62 nm) on 14 cm and the average diameter is 141 nm (SD. is 40 nm) on 16 cm collection distance. Besides; the bead-on-string structure is not obtained and PVA nanofiber distribute uniformly on the nanofiber mat.