Abstract: The enzymatic treatments based on the combined use of cutinase and protease (alkaline protease of Savinase or papain) treatments were contrastively studied. The effects the two-step combined treatments on wool anti-felting were investigated. The results indicated that Savinase treatment was more efficient than papain treatment during wool processing, it led to more weight loss after wool enzymatic incubating. For the wool fabric treated with papain, when the weight loss was similar to that of the sample treated with Savinase, the wettability of the former was more satisfactory, the alkaline solubility and shrinkage percentage were also appreciably lower, revealing that the extent of scale removal was more remarkable during cutinase-papain treatment. The data obtained by the XPS method further showed that papain seems to degrade the scales more efficiently in hydrolysis mode than the protease of Savinase during wool enzymatic processing.