Abstract: MTT assays is adopted to identify of inhibiting effect of two kinds of N,N-dialkyl urea compounds and four kinds of alkyphenol ethoxylates surfactants on HeLa cells and CHO cells ,and to explore two types of material cytotoxicity. The results indicated that the reproduction restraining effect of N,N-dialkyl urea and alkyphenol ethoxylates on HeLa cells and CHO cells gradually increased with concentration increased. N,N-diethyl urea and N,N-dipropyl urea could inhibit proliferation of HeLa cells and CHO cells at the concentration of 10mg/ml, and the inhibition rate were more than 80%. Alkyphenol ethoxylates OP-7、OP-9、NP-10 and NP-25 could inhibit proliferation of HeLa cells and CHO cells at the concentration of 0.1mg/ml、0.1mg/ml、0.1mg/ml and 0.5mg/ml, and the inhibition rate were more than 80%. Therefore, when the six substances reach a certain concentration, they inhibits the growth of HeLa cells and CHO cells significantly., and shows a dose-dependent manner. N,N-dialkyl urea shows less toxicity than alkyphenol ethoxylates.

Key words: N,N-dialkyl urea , alkylphenol ethoxylate , CHO cell , HeLa cell , MTT-assay