Abstract: To establish a method for the simultaneous determination of seven allergenous disperse dyestuffs, such as Disperse Yellow 1,Disperse Yellow 3, Disperse Yellow 9, Disperse Yellow 49, Disperse Red 1, Disperse Red 11, Disperse Red 17 in polyester sewing thread by high-performance liquid chromatography with ultraviolet detection. After the object analytes were extracted by methanol, the sample was separated on a C18 column by a gradient elution with acetonitrile- formic acid (0.1%)- ammonium acetate (0.02 mol/L) as the mobile phase. Detection was carried out by ultraviolet detector at 360 nm,440 nm and 480 nm. The extraction recoveries were between 90.0%~101.0%, and the RSDs were between 1.0%~ 5.4%. Calibration curves of the seven disperse dyestuffs were linear within the range of 1.0 mg/L ~50.0 mg/L with correlation coefficients of more than 0.999. The limits of detection (LOD) were between 0.2 mg/kg~1.0 mg/kg. This established method is simple, sensitive, accurate and good specificity for monitoring of the seven allergenous disperse dyestuffs in polyester sewing thread.

Key words: liquid chromatography , polyester sewing thread , allergenous disperse dydstuffs , ultravtolet detection