Abstract: Abstract: Mixing tetracycline hydrochloride (tet) with poly-(D,L-lactide-co-glycolide) (PLGA) in THF and DMF (1:1 v/v),the PLGA nanofibers membrane with tetracycline hydrochloride were prepared by electrospinning. The morphlogy were observed by means of scan electric microscope(SEM) and fluorescence microscopy. The absorbency of drug was detected by UV-visible spectroscopy, then counting the velocity of drug release. The antimicrobial activity in vitro were examined by improved Kirby-Bauer diffusion method.Cells and scaffold of the morphology, adhesion and growth were observed by SEM. The average diameter of PLGA nanofibers with different tet contents between 360nm and 470nm . The 10% drug loaded membranes exhibited the highest initial burst release. Tet release sustained over 27 days in vitro. The antimicrobial activity in vitro was effective in inhibiting Staphylococcus aureus growth. Examination of the biocompatibility of PLGA/tet scaffolds with MG-63 cells showed no adverse effect on the cell adhesion and proliferation. So PLGA/tet nanofibers has good biocompatibility.