Abstract: To investigate the interaction between silk peptide and reactive orange K-7R(RK-7R), multiple spectroscopy (Ultraviolet visible, fluorescence spectroscopy, synchronous fluorescence, Rayleigh resonance spectrum have been used. The results showed that a non fluorescence complex was formed between silk peptide and RK-7R. The binding distance and binding ratio were calculated as 3.46nm and 1:1, respectively. The binding constants increased when ionic liquids including chloride hydroxyethyl trimethyl amine, bromide 1-ethyl-2 ,3–dimethyl imidazolium, 1-ethyl-2,3-methyl imidazolium bis-[trifluoromethyl-sulfonyl] imide, metal ions (Ca2 +, Pb2 +, Ba2 +), and surfactants CTAB were added into SP- RK-7R Ssystem ,while decreased when Cu2+ and Triton X-100 were in the presence.. Sodium dodecyl sulfate had no effects on the system.

Key words: silk peptide, Reactive Orange K-7R, specteosopy, interaction