Abstract:

To improve the exploitation and application of ramie fiber, a set of method for the composition analysis of ramie single sugars was established. The high performance liquid chromatography (HPLC) coupling with pre-column derivatization by 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyze the monosaccharides in ramie. The samples were hydrolyzed by trifluoroacetic acid, then analyzed by HPLC with C18 column and ultraviolet detector. All monosaccharides possessed good linear relationship with the precision below 2.02% and recoveries between 96.53% and 102.76%. The results showed that the hydrolysis rate of ramie was 90%, the main monosaccharides were mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and few unknown monosaccharides. The glucose was the main constituent, with the proportion of 83.86%～86.54% of the known monosaccharides. The HPLC method coupling with pre-column derivatization by PMP can meet the detection requirements of instrument, and is suitable for the analysis of the hydrolysed monosaccharides in ramie.