Journal of Textile Research ›› 2019, Vol. 40 ›› Issue (07): 13-18.doi: 10.13475/j.fzxb.20180705106

• Fiber Materials • Previous Articles     Next Articles

Preparation and properties of regenerated silk fibroin/acellular dermal matrix blended nanofiber membrane

LIN Yongjia1, YANG Dongchao2, ZHANG Peihua1(), GU Yan2   

  1. 1. Key Laboratory of Textile Science & Technology of Ministry of Education, Donghua University, Shanghai 201620, China;
    2. Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Received:2018-07-20 Revised:2019-04-10 Online:2019-07-15 Published:2019-07-25
  • Contact: ZHANG Peihua E-mail:phzh@dhu.edu.cn

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Abstract:

In order to further improve the application potential of regenerated silk fibroin (RSF) in biological tissue engineering, RSF and acellular dermal matrix (ADM) were dissolved in formic acid in different mass ratios to prepare a spinning solution for electrospinning. The morphology, microstructure and biocompatibility of nanofibers were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry and CCK8 kit. The test results show that at a fixed mass ratio of 9∶1, the nanofibers spun at a mass concentration of 13 g/mL are more regular, and the spinning state is more stable. When the fixed spinning solution mass concentration is 13 g/mL, as the proportion of ADM in the blend increases, the viscosity of the spinning solution increases gradually, the spinnability deteriorates, and the fiber morphology gradually becomes irregular. An interaction exists between ADM and RSF. Some of the random structures in the blended nanofiber membrane gradually shift to the β-sheet structure. The RSF/ADM blended nanofiber membrane has good biocompatibility.

Key words: biomedical material, electrospinning, regenerated silk fibroin, acellular dermal matrix, biocompatibility

CLC Number: 

  • TS101.4

Fig.1

Morphologies of different mass concentrations of RSF/ADM blended nanofibers(×3 000)"

Tab.1

Different mass concentrations of RSF/ADM blended nanofibers in diameter"

质量浓度/(g·mL-1) 平均直径/nm CV值/%
9 358 41.6
11 462 24.5
13 616 30.4
15 685 35.6

Fig.2

Morphologies of nanofibers with different blending ratios (×3 000)"

Tab.2

Diameter of RSF/ADM nanofibers with different blend ratios"

RSF与ADM质量比 平均直径/nm CV值/%
10∶0 618 19.0
9∶1 616 30.4
8∶2 507 38.1
7∶3 498 36.3
6∶4 499 24.8
5∶5 - -

Fig.3

Rheological curves of different proportions of RSF/ADM blend spinning solution"

Fig.4

Infrared spectra of nanofiber membranes with different blending ratioes"

Fig.5

DSC curves a with different blending ratioes of nanofiber membranes"

Tab.3

OD value of cells after culture on different substrates"

时间/d OD值 P值
共混纳米纤维膜 空白
1 0.771±0.083 0.859±0.031 0.135
3 1.660±0.023 1.311±0.045 <0.01
5 2.644±0.059 2.552±0.028 0.051
7 2.823±0.052 2.829±0.039 0.878
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