Journal of Textile Research ›› 2019, Vol. 40 ›› Issue (10): 7-12.doi: 10.13475/j.fzxb.20181003306

• Fiber Materials • Previous Articles     Next Articles

Preparation and antibacterial activity of silk fibroin/chitosan microspheres

ZHANG Zhibin1,2, LI Gang1,2(), MAO Senxian3, LI Xunxun3, CHEN Yushuang3, MAO Qingshan3, LI Yi4, PAN Zhijuan1,2, WANG Xiaoqin1,2   

  1. 1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
    2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
    3. Zhejiang Truelove Vogue Co., Ltd., Jinhua, Zhejiang 322000, China
    4. School of Materials, The University of Manchester, Manchester M139PL, UK
  • Received:2018-10-18 Revised:2019-05-11 Online:2019-10-15 Published:2019-10-23
  • Contact: LI Gang E-mail:tcligang@suda.edu.cn

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Abstract:

In order to develop a natural and harmless antibacterial finishing agent which is beneficial to human body, silk fibroin/chitosan microspheres were prepared by emulsion polymerization. The microscopic morphology, chemical structure, thermal stability and antibacterial properties of the microspheres with different ratios of silk fibroin and chitosan were examined. The results show that the silk fibroin mass fraction is 6%, the chitosan mass fraction is 3%, and the volume ratio of chitosan to silk fibroin is 1∶2, the microspheres have a spherical shape and smooth surface with particle size between 0.4 to 1.4 μm. In addition, it has a good antibacterial effect against Escherichia coli and Staphylococcus aureus, with the antibacterial ratio of(82 ± 4.2)% and(80 ± 2.6)%, respectively. The secondary structure of the microspheres changes from silk I to silk II, and the initiation temperature of thermal decomposition is higher than 250 ℃, showing good thermal stability.

Key words: silk fibroin, chitosan, microsphere, antibacterial property, emulsion polymerization

CLC Number: 

  • TS141.8

Fig.1

SEM images of microspheres with different volume ratios of CS and SF"

Fig.2

X-ray diffraction of microspheres with different volume ratios of CS and SF"

Fig.3

FT-IR spectra of CS, SF and CS/SF microspheres"

Fig.4

TG curves of CS, SF and CS/SF microspheres"

Fig.5

Antibacterial effects of SF(a)and CS/SF(b) microspheres on Escherichia coli"

Fig.6

Antibacterial effects of SF(a)and CS/SF(b) microspheres on Staphylococcus aureus"

Fig.7

Antibacterial properties of microspheres with different volume ratios of CS and SF"

Fig.8

Formation mechanism of SF/CS microspheres"

[1] 崔立华, 马辉, 徐德增. 用银离子沸石改性PET抗菌纤维的研究[J]. 聚酯工业, 2010,23(2):18-21.
CUI Lihua, MA Hui, XU Dezeng. Study on modification of PET antibacterial fiber with silver ion zeolite[J]. Polyester Industry, 2010,23(2):18-21.
[2] 邢彦军, 宋阳, 吉友美, 等. 银系抗菌纺织品的研究进展[J]. 纺织学报, 2008,29(4):127-133.
XING Yanjun, SONG Yang, JI Youmei, et al. Advances in antimicrobial finishing for textiles based on silver[J]. Journal of Textile Research, 2008,29(4):127-133.
[3] MAI Y J, LUO H, ZHAO X Y, et al. Organosilicon functionalized quaternary ammonium ionic liquids as electrolytes for lithium-ion batteries[J]. Ionics, 2014,20(9):1207-1215.
doi: 10.1007/s11581-014-1085-4
[4] ZHANG Q, LU S, ZHENG A, et al. Preparation and characterization of long-acting antimicrobial polyethylene terephthalate via covalent bonding method[J]. Chemical Journal of Chinese Universities (Chinese Edition), 2014,35(4):873-880.
[5] CHANG Y B, TU P C, WU M W, et al. A study on chitosan modification of polyester fabrics by atmospheric pressure plasma and its antibacterial effects[J]. Fibers & Polymers, 2008,9(3):307-311.
[6] LIM S H, HUDSON S M. Synjournal and antimicrobial activity of a water-soluble chitosan derivative with a fiber-reactive group[J]. Carbohydrate Research, 2004,339(2):313-319.
doi: 10.1016/j.carres.2003.10.024 pmid: 14698889
[7] NELSON G. Microencapsulation in textile finishing[J]. Coloration Technology, 2010,31(1):57-64.
doi: 10.1111/cote.2001.31.issue-1
[8] BRUGNEROTTO J, LIZARDI J, GOYCOOLEA F M, et al. An infrared investigation in relation with chitin and chitosan characterization[J]. Polymer, 2001,42(8):3569-3580.
doi: 10.1016/S0032-3861(00)00713-8
[9] HAN Y P, KE-YI L I, YANG H W, et al. Research on antibacterial mechanism of chitosan and its chemical modification[J]. Chemical World, 2012,16(4):248-252.
[10] ANITHA A, RANI V V D, KRISHNA R, et al. Synjournal, characterization, cytotoxicity and antibacterial studies of chitosan, carboxymethyl and, carboxymethyl chitosan nanoparticles[J]. Carbohydrate Polymers, 2009,78(4):672-677.
doi: 10.1016/j.carbpol.2009.05.028
[11] JIANG C, WANG X, GUNAWIDJAJA R, et al. Mechanical properties of robust ultrathin silk fibroin films[J]. Advanced Functional Materials, 2010,17(13):2229-2237.
doi: 10.1002/adfm.v17:13
[12] WEI Z, GU Z. A study of one-bath alkali-amine hydrolysis and silk-fibroin finishing of polyester microfiber crepe fabric[J]. Journal of Applied Polymer Science, 2001,81(6):1467-1473.
doi: 10.1002/(ISSN)1097-4628
[13] SOFIA S, MCCARTHY M B, GRONOWICZ G, et al. Functionalized silk-based biomaterials for bone formation[J]. Journal of Biomedical Materials Research, 2001,54(1):139-148.
doi: 10.1002/1097-4636(200101)54:1<139::aid-jbm17>3.0.co;2-7 pmid: 11077413
[14] LIU J, LIU C, LIU Y, et al. Study on the grafting of chitosan-gelatin microcapsules onto cotton fabrics and its antibacterial effect[J]. Colloids & Surfaces Biointerfaces, 2013,109(9):103-108.
[15] LI M, LU S, WU Z, et al. Study on porous silk fibroin materials: I: fine structure of freeze dried silk fibroin[J]. Journal of Applied Polymer Science, 2015,79(12):2185-2191.
doi: 10.1002/(ISSN)1097-4628
[16] WU J, XIE X, ZHENG Z, et al. Effect of pH on polyethylene glycol (PEG)-induced silk microsphere formation for drug delivery[J]. Materials Science & Engineering C: Materials for Biological Applications, 2017,80:549-557.
doi: 10.1016/j.msec.2017.05.072 pmid: 28866200
[17] LEFEVRE T, RPUSSEAU M E, PEZOLET M. Protein secondary structure and orientation in silk as revealed by Raman spectromicroscopy[J]. Biophysical Journal, 2007,92(8):2885-2895.
doi: 10.1529/biophysj.106.100339 pmid: 17277183
[18] LÜ J, ZHO Q, ZHI T, et al. Environmentally friendly surface modification of polyethylene terephthalate (PET) fabric by low-temperature oxygen plasma and carboxymethyl chitosan[J]. Journal of Cleaner Production, 2016,118:187-196.
doi: 10.1016/j.jclepro.2016.01.058
[19] 杨俊玲. 壳聚糖抗菌性的研究[J]. 纺织学报, 2003,24(2):151-153.
YANG Junling. Study on the antibacterial activity of chitosan[J]. Journal of Textile Research, 2003,24(2):151-153.
[20] 吴建兵. 丝素纳微米球的制备及其在药物控释方面的应用研究[D]. 苏州:苏州大学, 2017: 41-44.
WU Jianbin. Development of novel silk nano-microspheres for controlled drug release appli-cations[D]. Suzhou: Soochow University, 2017: 41-44.
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