Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (02): 13-19.doi: 10.13475/j.fzxb.20190102807

• Fiber Materials • Previous Articles     Next Articles

Preparation and properties of silk fibroin based bilayer dressing materials

ZHONG Hongrong, FANG Yan, BAO Hong, WU Tingfang, ZHANG Xiaoning, XU Shui, ZHU Yong()   

  1. College of Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2019-01-11 Revised:2019-10-24 Online:2020-02-15 Published:2020-02-21
  • Contact: ZHU Yong E-mail:zhuy@swu.edu.cn

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Abstract:

In order to solve poor water absorption and insufficient mechanical properties of silk fibroin (SF), the SF/Glu sponge was prepared through lyophilization with silk fibroin as the substrate and hydrophilic glucose (Glu) and plasticizer glycerol (Gly) as the auxiliary materials. In the meantime, polyurethane (PU) was manufactured into membrane by using the evaporation solvent method. A medical hot melt adhesive was then employed to glue the SF/Glu sponge and PU membrane together to create the bilayer dressing materials, whose structure and properties were analyzed afterwards. The results show that when the mass fraction of Gly is set to 0.5%, that of PU to 8%, and that of Glu to 10%, the water absorption by silk fibroin bilayer dressing material became 12.5 times higher than its own mass. In addition, the bilayer dressing demonstrates a low dissolubility of less than 2% and the water retention time reaches 11 h. Structural characterization reveals that the glucose and silk fibroin have good compatibility and glucose enables silk fibroin to maintain a stable β-sheet structure. The bilayer dressing bio-safety experiments further indicate that the new dressing material is not cytotoxic and is bacterial-resistant.

Key words: silk fibroin, bilayer dressing, glucose, polyurethane membrane, bio-safety

CLC Number: 

  • TQ341.5

Fig.1

Water absorption of bilayer dressing composed of glucose with different mass fraction"

Fig.2

Dissolubility of bilayer dressing composed of glucose with different mass fraction"

Fig.3

Water retention of bilayer dressing composed of glucose with different mass fraction"

Fig.4

Water vapor transmission ratio of bilayer dressing composed of glucose with different mass fraction"

Fig.5

SEM images of polyurethane membrane and glucose sponge with different mass fraction. (a) PU membrane; (b) 0; (c) 5%; (d) 10%;(e) 15%; (f) 20%"

Fig.6

FT-IR spectra of glucose"

Fig.7

FT-IR spectra of SF and with different mass fraction Glu"

Fig.8

DSC curve of SF and sponge with different mass fraction Glu"

Fig.9

Resistance bacteria results of sterile gauzeand and polyurethane membrane. (a)Sterile gauze (Escherichia coli 106); (b) Polyurethane membrane (Escherichia coli 104); (c) Sterile gauze (Staphyloccocus aureus 106); (d) Polyurethane membrane (Staphyloccocus aureus 104)"

Tab.1

Relatively growth rates of bilayer dressing with different ratio%"

Glu质量分数 1 d 3 d 5 d
0 117.72 104.54 100.48
5 123.59 109.67 104.49
10 128.85 111.58 108.50
15 126.89 111.79 105.53
20 123.59 108.73 102.81
阳性对照 16.13 7.58 7.19
阴性对照 100.00 100.00 100.00
[1] 许宗溥, 杨明英, 潘彩霞, 等. 丝素创伤敷料的制备与应用研究进展[J]. 蚕业科学, 2015,41(2):376-380.
XU Zongpu, YANG Mingying, PAN Caixia, et al. Research progress in preparation and application of silk fibroin wound dressings[J]. Science of Sericulture, 2015,41(2):376-380.
[2] 卢志华, 马育栋, 侯宗良, 等. 丝素蛋白/壳聚糖敷料对大鼠皮肤再生作用的实验研究[J]. 济宁学院学报, 2015,29(3):27-30.
LU Zhihua, MA Yudong, HOU Zongliang, et al. Application of silk fibroin/chitosan dressing to rat skin regeneration[J]. Journal of Jining University, 2015,29(3):27-30.
[3] BAIMARK Y, SRIHANAM P, SRISUWAN Y. Preparation of flexible silk fibroin films plasticized with glucose[J]. Asian Journal of Materials Science, 2009,1(1):29-35.
[4] SRIVASTAVA C M, PURWAR R, KANNAUJIA R, et al. Flexible silk fibroin films for wound dressing[J]. Fibers & Polymers, 2015,16(5):1020-1030.
[5] SRIVASTAVA C M, PURWAR R, GUPTA A, et al. Dextrose modified flexible tasar and muga fibroin films for wound healing applications[J]. Materials Science & Engineering C, 2017,75:104-114.
doi: 10.1016/j.msec.2017.02.021 pmid: 28415387
[6] PANICO A, PALADINI F, POLLINI M. Development of regenerative and flexible fibroin-based wound dressings[J]. Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 2018,1:1-12.
[7] REED A M. Mitraflex: development of an intelligent, spyrosorbent wound dressing[J]. Journal of Biomaterials Applications, 1991,6(1):3-41.
doi: 10.1177/088532829100600101 pmid: 1920068
[8] ANDREWS W L, HAMMETT R C. Wound dressing for the hands: US5328449[P]. 1994-07-12.
[9] 徐水, 张胡静, 李雯静, 等. 丝素/纳米SiO2凝胶共混膜的制备及性能测试[J]. 蚕业科学, 2011,37(1):82-87.
XU Shui, ZHANG Hujing, LI Wenjing, et al. Preparation and property determination of fibroin/nano-SiO2 gel blend membrane[J]. Science of Sericulture, 2011,37(1):82-87.
[10] GE Tichi, XING Nan, CHEN Jiong, et al. Comparison among several foam dressings in the properties of water-absorption, water-locking and air permeability[J]. Chinese Journal of Burns, 2012,28(5):349-352.
pmid: 23290760
[11] ZHANG Peng, WANG Wenliang. Preparation of silk fibroin-chitosan scaffolds and their properties[J]. Chinese Journal of Reparative and Reconstructive Surgery, 2013,27(12):1517-1522.
pmid: 24640377
[12] 郑丽, 朱平, 张林, 等. 纤维素/丝素蛋白共混膜的制备及性能研究[J]. 印染助剂, 2012,29(6):27-29.
ZHENG Li, ZHU Ping, ZHANG Lin, et al. Study on the preparation and performance of cellulose/silk fibroin composite film[J]. Textile Auxiliaries, 2012,29(6):27-29.
[13] SIRITIENTONG T, RATANAVARAPORN J, SRICHANA T, et al. Preliminary characterization of genipin-cross-linked silk sericin/poly(vinyl alcohol) films as two-dimensional wound dressings for the healing of superficial wounds[J]. Biomed Research International, 2013(6):1-13.
[14] 翁诗甫. 傅里叶变换红外光谱仪[M]. 北京:化学工业出版社, 2010: 101.
WENG Shifu. Fourier transform infrared spectro-meter[M]. Beijing:Chemical Industry Press, 2010: 101.
[15] MALIN S F, RUCHTI T L, BLANK T B, et al. Noninvasive prediction of glucose by near-infrared diffuse reflectance spectroscopy[J]. Clinical Chemistry, 1999,45(9):1651-1658.
pmid: 10471679
[16] 李震. 差示扫描量热法(DSC)在化学中的应用[J]. 泰山师专学报, 2000(6):32-34.
LI Zhen. Application of differential scanning calori-metry (DSC) in chemistry[J]. Journal of Taian Teachers College, 2000(6):32-34.
[17] 刘春波, 申钦鹏, 杨光宇, 等. 热分析-傅里叶变换红外光谱-气相色谱-质谱联用测定葡萄糖的热分解产物[J]. 理化检验-化学分册, 2014,50(11):1342-1347.
LIU Chunbo, SHEN Qinpeng, YANG Guangyu, et al. Determination of thermal decomposition products of glucose by thermal analysis combined with FTIR and GC-MS[J]. PTCA(Part B: Chemical Analysis), 2014,50(11):1342-1347.
[18] SHANKAR S, WANG L F, RHIM J W. Preparations and characterization of alginate/silver composite films: Effect of types of silver particles[J]. Carbohydrate Polymers, 2016,146:208-216.
pmid: 27112867
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