Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (02): 13-19.doi: 10.13475/j.fzxb.20190102807

• Fiber Materials • Previous Articles     Next Articles

Preparation and properties of silk fibroin based bilayer dressing materials

ZHONG Hongrong, FANG Yan, BAO Hong, WU Tingfang, ZHANG Xiaoning, XU Shui, ZHU Yong()   

  1. College of Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2019-01-11 Revised:2019-10-24 Online:2020-02-15 Published:2020-02-21
  • Contact: ZHU Yong E-mail:zhuy@swu.edu.cn

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Abstract:

In order to solve poor water absorption and insufficient mechanical properties of silk fibroin (SF), the SF/Glu sponge was prepared through lyophilization with silk fibroin as the substrate and hydrophilic glucose (Glu) and plasticizer glycerol (Gly) as the auxiliary materials. In the meantime, polyurethane (PU) was manufactured into membrane by using the evaporation solvent method. A medical hot melt adhesive was then employed to glue the SF/Glu sponge and PU membrane together to create the bilayer dressing materials, whose structure and properties were analyzed afterwards. The results show that when the mass fraction of Gly is set to 0.5%, that of PU to 8%, and that of Glu to 10%, the water absorption by silk fibroin bilayer dressing material became 12.5 times higher than its own mass. In addition, the bilayer dressing demonstrates a low dissolubility of less than 2% and the water retention time reaches 11 h. Structural characterization reveals that the glucose and silk fibroin have good compatibility and glucose enables silk fibroin to maintain a stable β-sheet structure. The bilayer dressing bio-safety experiments further indicate that the new dressing material is not cytotoxic and is bacterial-resistant.

Key words: silk fibroin, bilayer dressing, glucose, polyurethane membrane, bio-safety

CLC Number: 

  • TQ341.5

Fig.1

Water absorption of bilayer dressing composed of glucose with different mass fraction"

Fig.2

Dissolubility of bilayer dressing composed of glucose with different mass fraction"

Fig.3

Water retention of bilayer dressing composed of glucose with different mass fraction"

Fig.4

Water vapor transmission ratio of bilayer dressing composed of glucose with different mass fraction"

Fig.5

SEM images of polyurethane membrane and glucose sponge with different mass fraction. (a) PU membrane; (b) 0; (c) 5%; (d) 10%;(e) 15%; (f) 20%"

Fig.6

FT-IR spectra of glucose"

Fig.7

FT-IR spectra of SF and with different mass fraction Glu"

Fig.8

DSC curve of SF and sponge with different mass fraction Glu"

Fig.9

Resistance bacteria results of sterile gauzeand and polyurethane membrane. (a)Sterile gauze (Escherichia coli 106); (b) Polyurethane membrane (Escherichia coli 104); (c) Sterile gauze (Staphyloccocus aureus 106); (d) Polyurethane membrane (Staphyloccocus aureus 104)"

Tab.1

Relatively growth rates of bilayer dressing with different ratio%"

Glu质量分数 1 d 3 d 5 d
0 117.72 104.54 100.48
5 123.59 109.67 104.49
10 128.85 111.58 108.50
15 126.89 111.79 105.53
20 123.59 108.73 102.81
阳性对照 16.13 7.58 7.19
阴性对照 100.00 100.00 100.00
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