Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (09): 167-173.doi: 10.13475/j.fzxb.20200307807

• Column: Biomedical Textile Materials and It′s Products • Previous Articles     Next Articles

Preparation of polycaprolactone/polyethylene glycol nanofiber membranes with large pore sizes and its application for tissue engineering scaffold

PAN Lu1, CHENG Tingting1, XU Lan1,2()   

  1. 1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
    2. National Engineering Laboratory for Modern Silk, Suzhou, Jiangsu 215123, China
  • Received:2020-03-30 Revised:2020-06-04 Online:2020-09-15 Published:2020-09-25
  • Contact: XU Lan E-mail:lanxu@suda.edu.cn

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Abstract:

In order to improve the growth and adhesion of cells on electrospun nanofiber scaffolds, a modified electrospinning (ES) device was used to prepare polycaprolactone (PLC)/polyethylene glycol (PEG) composite nanofiber membranes (CNFMs) with large pore sizes, aiming for good biocompatibility. The influences of the composition and mass fraction of spinning solution on the morphology and properties of the CNFMs were investigated, and the optimal spinning parameters were determined. The CNFMs obtained using the modified ES under the optimal conditions were taken as tissue engineering scaffold, and the cytocompatibility of the CNFMs was compared with that of the CNFMs prepared by the traditional ES. The results show that when the blend mass ratio of PLC and PEG is 80∶20 and the mass fraction of spinning solution is 25%, the quality of PCL/PEG CNFMs with large pore sizes is the best. Moreover, compared with the PCL/PEG CNFMs obtained by the traditional ES, the PCL/PEG CNFMs with large pore sizes supply a more conducive environment for the cell growth and proliferation, and are more suitable to be used as tissue engineering scaffold materials.

Key words: electrospinning, nanofiber membranes with large pore sizes, tissue engineering scaffold, polycaprolactone, polyethylene glycol

CLC Number: 

  • TS131.9

Fig.1

Schematic of modified electrospinning device"

Fig.2

SEM images of nanofiber membranes with different mass ratios of PEG and PCL(×10 000)"

Tab.1

Diameter statistics of nanofibers with different mass ratios of PEG and PCL"

PCL与PEG的质量比 平均直径/nm 标准差/nm 置信区间/nm
30:70 187.30 82.88 ±16.24
50:50 223.14 81.45 ±15.96
70:30 306.55 135.39 ±26.54
80:20 336.40 139.66 ±27.37
90:10 364.01 295.17 ±57.85

Tab.2

Mechanical properties of nanofiber membranes with different mass ratios of PEG and PCL"

PCL与PEG的质量比 断裂强度/MPa 断裂伸长率/%
30:70 0.62 25.13
50:50 0.90 57.13
70:30 1.51 83.80
80:20 2.46 87.15
90:10 3.71 92.43

Fig.3

Morphology of nanofibers with different PCL/PEG concentrations(×10 000)"

Tab.3

Diameter statistics of nanofibers with different PCL/PEG mass fractions"

溶液质量分数/% 平均直径/nm 标准差/nm 置信区间/nm
15 130.56 52.01 ±10.19(微球)
20 168.63 86.62 ±16.98(微球)
25 335.95 136.41 ±26.74
30 340.23 138.19 ±27.08

Tab.4

Mechanical properties of nanofiber membranes with different PCL/PEG mass fractions"

溶液质量分数/% 断裂强度/MPa 断裂伸长率/%
15 0.31 68.91
20 0.81 85.15
25 2.46 87.14
30 2.78 93.40

Fig.4

SEM images of traditional (a) and modified (b)electrospinning PCL/PEG membranes(×2 000)"

Tab.5

Pore size statistics of traditional and modified electrospinning PCL/PEG membranes"

纺丝方式 平均孔面积/μm2 标准差/μm2 置信区间/μm2
传统静电纺 9.00 2.94 ±0.41
改进静电纺 15.22 3.59 ±0.50

Fig.5

Adhesion (a) and proliferation (b) of EC on nanofiber membranes"

Fig.6

SEM images of EC cultured on traditional(a)and improved(b)nanofiber membranes at different times(×600)"

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