Journal of Textile Research ›› 2021, Vol. 42 ›› Issue (08): 90-95.doi: 10.13475/j.fzxb.20200808206

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Optimization and characterization of flavonoids extracted from jute fiber

CUI Qilu1,2, LI Jiawei1, PAN Liugui1, YANG Fei1,3, YU Chongwen1,2()   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
    3. Zhejiang Golden Eagle Co., Ltd., Zhoushan, Zhejiang 316051, China
  • Received:2020-08-20 Revised:2021-05-06 Online:2021-08-15 Published:2021-08-24
  • Contact: YU Chongwen E-mail:yucw@dhu.edu.cn

Abstract:

In order to confirm the existence of flavonoids in jute fiber and to develop the application value, the jute fiber extracts were obtained using alcohol-water method. The content of flavonoids in the extraction solution was tested using ultraviolet spectrophotometer. The extraction process was optimized based on the use of quadratic general rotary design, and the relationships between the three factors(alcohol volume fraction, bath ratio and extracting time) and the extraction rate of flavonoids were studied. The chemical structure, thermal stability and morphology of jute fiber extracts after rotary evaporation and vacuum freeze drying were tested and analyzed. The results show that the chemical structure of jute fiber extracts is similar to the structure of rutin standard, which indicates that it contains flavonoids. The optimal extraction conditions were found to be ethanol volume fraction 75%, bath ratio 1∶50, and extracting time 150 min, at which the extraction rate of flavonoids was 0.037%.

Key words: jute fiber, flavonoid, responsive surface analysis, extraction process, alcohol-water method

CLC Number: 

  • TS121

Tab.1

Factors coding in experiment"

水平 A
乙醇体积分数x1/%
B
浴比x2
C
时间x3/min
-1.682(-γ) 60 1∶30 60
-1 64 1∶34 78
0 70 1∶40 105
1 76 1∶46 132
+1.682(+γ) 80 1∶50 150

Tab.2

Experiment design and extraction rate for each test"

试验号 x0 A B C 提取率ye/%
1# 1 1 1 1 0.007 8
2# 1 1 1 -1 0.012 2
3# 1 1 -1 1 0.012 2
4# 1 1 -1 -1 0.014 7
5# 1 -1 1 1 0.013 3
6# 1 -1 1 -1 0.013 3
7# 1 -1 -1 1 0.007 4
8# 1 -1 -1 -1 0.018 3
9# 1 1.682 0 0 0.004 9
10# 1 -1.682 0 0 0.003 9
11# 1 0 1.682 0 0.006 1
12# 1 0 -1.682 0 0.018 6
13# 1 0 0 1.682 0.008 7
14# 1 0 0 -1.682 0.020 1
15# 1 0 0 0 0.012 5
16# 1 0 0 0 0.023 0
17# 1 0 0 0 0.014 4
18# 1 0 0 0 0.015 3
19# 1 0 0 0 0.007 7
20# 1 0 0 0 0.017 2

Tab.3

Regression coefficient estimation and significance test result"

项目 数值 t检验 显著性
b0 0.013 2.182 476>t0.1(10) 显著
b1 -0.003 654 0.924 61<t0.1(10) 不显著
b2 -0.002 69 0.680 67<t0.1(10) 不显著
b3 -0.003 974 1.005 57<t0.1(10) 不显著
b12 -0.002 708 1.658 43<t0.1(10) 不显著
b13 0.006 855 4.197 795>t0.01(10) 显著
b23 0.004 458 2.799 45>t0.02(10) 显著
b11 -0.008 6 2.235 4>t0.05(10) 显著
b22 -0.006 5 1.689 63<t0.1(10) 不显著
b33 0.002 561 0.665 714> t0.1(10) 显著

Tab.4

Significance test result of regression equation"

指标 F1 F2 Fα1 Fα2 显著性
提取率 0.66 1.68 F0.01(5,5)=11.0 F 0 . 25(9,10)=1.56 显著

Fig.1

Response of different parameters and their interaction on extraction rate of flavonoid. (a) Alcohol volume fraction and bath ratio;(b) Alcohol volume fraction and extraction time;(c) Extration time and bath ratio"

Tab.5

Validation test of optimal process"

试验号 因子 指标
y/%
x1/% x2 x3/min
8# 64 1∶34 78 0.018 3
12# 70 1∶30 105 0.018 6
14# 70 1∶40 60 0.020 1
16# 70 1∶40 105 0.023 0
最优解 预测值 75 1∶50 150 0.036 1
实测值 75 1∶50 150 0.037 0
较优解1 预测值 75 1∶47 150 0.032 3
实测值 75 1∶47 150 0.019 8
较优解2 预测值 75 1∶46 150 0.031 5
实测值 75 1∶46 150 0.018 0

Fig.2

FT-IR spectra of rutin and jute fiber extracts"

Fig.3

Surface SEM image of jute fiber extracts (×300)"

Tab.6

Element composition and content of jute fiber extracts"

元素 质量百分比/% 标准偏差
C 69.81 0.90
O 24.87 0.84
Na 0.43 0.11
Si 2.14 0.14
Cl 1.11 0.14
K 0.67 0.14
Ca 0.97 0.18

Fig.4

Thermal property of jute fiber extracts. (a) DSC curves; (b) TG and DTG curves"

[1] 张蓓蓓. 植物黄酮类化合物的研究[J]. 科技视野, 2018(23):155-157.
ZHANG Beibei. Study on plant flavonoids[J]. Science & Technology Vision, 2018(23):155-157.
[2] 刘一杰, 薛永常. 植物黄酮类化合物的研究进展[J]. 中国生物工程杂志, 2016, 36(9):81-86.
LIU Yijie, XUE Yongchang. The research progress of flavonoids in plants[J]. China Biotechnology, 2016, 36(9):81-86.
[3] 高世会, 郁崇文. 罗布麻中黄酮的超临界CO2萃取及其抗菌性[J]. 纺织学报, 2018, 39(8):71-76,82.
GAO Shihui, YU Chongwen. Supercritical carbon dioxide extraction and bacterial resistance of flavones from apocynum venetum bast fiber[J]. Journal of Textile Research, 2018, 39(8):71-76,82.
[4] 韩立杰, 李影, 刘子菱, 等. 榛花粉水和乙醇提取物抗氧化性的比较[J]. 食品科技, 2014, 39(2):224-227.
HAN Lijie, LI Ying, LIU Ziling, et al. Anti-oxidation and antimicrobial activity of water and ethanol extracts from hazel pollen[J]. Food Science and Technology, 2014, 39(2):224-227.
[5] 刘伯渠, 卢新生, 党金宁. 微波法提取红洋葱总黄酮的研究[J]. 吉林师范大学学报(自然科学版), 2018, 39(3):90-94.
LIU Boqu, LU Xinsheng, DANG Jinning. Research on the flavonoid extracting technology by microwave from red onion[J]. Journal of Jilin Normal Univer-sity (Natural Science Edition), 2018, 39(3):90-94.
[6] 陈建福. 响应面优化超声辅助提取黄槿叶总黄酮工艺及其亚硝酸盐清除能力[J]. 食品工业科技, 2019, 40(6):193-197,204.
CHEN Jianfu. Optimization of ultrasonic assisted extraction of total flavonoids from hibiscus tilisceus leaves by response surface methodology and its scavenging ability of nitrite[J]. Science and Technology of Food Industry, 2019, 40(6):193-197,204.
[7] 曾承露, 李锋. 酶法提取黄饭花黄酮工艺优化及其抗氧化活性研究[J]. 中国食品添加剂, 2017(6):93-98.
ZENG Chenglu, LI Feng. The optimization of flavonoids extraction from buddleja officinalis maxim by enzymatic and flavonoids antioxidant activity[J]. China Food Additives, 2017(6):93-98.
[8] 黄春梅. 菜用黄麻叶多糖、黄酮的萃取及菜用黄麻叶面条制作的研究[D]. 福州:福建农林大学, 2013: 20.
HUANG Chunmei. Studies on extract polysaccharide and total flavonoids from jute, and how to made jute noodle[D]. Fuzhou: Fujian Agriculture and Forest University, 2013: 20.
[9] 杨永涛. 罗布麻总黄酮的提取、分离纯化及抗氧化性能研究[D]. 广州:华南理工大学, 2018: 31.
YANG Yongtao. Study on extraction, purification and antioxidant activity of total flavonoids from apocynum venetum[D]. Guangzhou: South China University of Technology, 2018: 31.
[10] 陈如冰, 周雪娜, 陈婉芳, 等. 长蒴黄麻总黄酮的提取及其抗氧化性[J]. 贵州农业科学, 2011, 39(10):162-165.
CHEN Rubing, ZHOU Xuena, CHEN Wanfang, et al. Extraction and antioxidant activity of total flavonoids from corchorus olitorius[J]. Guizhou Agricultural Sciences, 2011, 39(10):162-165.
[11] 刘毅, 冷娟, 刘亮亮, 等. 大麻叶中总黄酮提取工艺的优化及其抗氧化活性测定[J]. 中国麻业科学, 2019, 41(1):13-23.
LIU Yi, LENG Juan, LIU Liangliang, et al. Optimization of extraction process for total flavonoids from hemp leaves and their antioxidant activity measure[J]. Plant Fiber Sciences in China, 2019, 41(1):13-23.
[12] 郁崇文, 汪军, 王新厚. 工程参数的最优化设计[M]. 上海: 东华大学出版社, 2003: 108.
YU Chongwen, WANG Jun, WANG Xinhou. Optimal design of engineering parameters[M]. Shanghai: Donghua University Press, 2003: 108.
[13] 林泽华. 侧柏叶黄酮和多糖分离纯化、结构表征及活性评价[D]. 广州:华南理工大学, 2016: 68.
LIN Zehua. Purification and structural characterization of flavonoids and polysaccharides from platycladus orientalis (L.) franco leaves and their biological activities[D]. Guangzhou: South China University of Technology, 2016: 68.
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