Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (12): 82-87.doi: 10.13475/j.fzxb.20211108006

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Optimizing microorganism degumming process of apocynum and bacterial screening

CHENG Fen1, ZHANG Xingqun1,2, WANG Yunlong1, WANG Ying1()   

  1. 1. College of Textile and Clothing, Xinjiang University, Urumqi, Xinjiang 830046, China
    2. College of Biological Sciences and Medical Engineering, Donghua University, Shanghai 201620, China
  • Received:2021-11-17 Revised:2022-07-23 Online:2022-12-15 Published:2023-01-06
  • Contact: WANG Ying E-mail:328532518@qq.com

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Abstract:

In order to improve the efficiency of apocynum degumming, dominant degumming strains was screened from the soil of the wild hemp growth area of Urumqi, Xinjiang using the transparent zones method, DNS colorimetry method and 16S rRNA sequencing. The superior degumming strains were used to optimize the microbial degumming process of apocynum by the orthogonal experiment designed in this study. The experimental results show that seven dominant degumming strains with higher pectinase, xylanase and low cellulase are theoretically suitable for practical applications of apocynum degumming, which belong to four genera including Bacillus, Enterobacter, Klebsiella and Pantoea. The optimal parameters optimized by orthogonal tests are initial pH 7 of the degumming solution, bath ratio of 1:40 and shaker speed of 90 r/min. Using the optimization process parameters, the residual gum rate of complex bacteria can be reduced to 30.45%, the degumming effect is improved and thus the best method is degumming with 7 strains, because a stable degumming colony is formed between strains among which the synergism and antagonism may affect the degumming ability.

Key words: apocynum, bacterial screening, enzyme activity, microbial degumming, degumming process

CLC Number: 

  • TS192.5

Tab.1

Configuration of sugar standard solutionmL"

试管号 糖标液体积 去离子水体积 DNS体积
1 0.0 2.0 2.0
2 0.2 1.8 2.0
3 0.4 1.6 2.0
4 0.5 1.5 2.0
5 0.6 1.4 2.0
6 0.7 1.3 2.0
7 0.8 1.2 2.0
8 1.0 1.0 2.0

Tab.2

Horizontal table of orthogonal test factors"

水平 A
摇床转速/(r·min-1)		 B
pH值		 C
浴比
1 0 7 1∶30
2 90 8 1∶40
3 150 9 1∶50

Fig.1

HC value of Congo Red hydrolytic circle"

Tab.3

Results of enzyme activity determinationU/mL"

菌株编号 果胶酶活 木聚糖酶活 纤维素酶酶活
Y8 117.565 9.390 2.825
X7 54.426 33.470 0.220
YE13 83.217 66.470 -
LT3 62.507 28.110 -
Z4 139.032 31.280 -
2-1 141.557 - -
X5 61.118 55.560 -
L1 95.340 50.600 -
GAN1 106.578 104.890 -

Tab.4

Results of gene sequence alignment"

菌株编号 细菌种属 GenBank登录号 相似性/%
2-1 芽孢杆菌属
(Bacillus subtilis subsp.)		 NR 112686.1 100.00
X7 克雷伯菌属
(Klebsiella sp.)		 MK 780166.1 100.00
YE13 芽孢杆菌属
(Bacillus subtilis subsp.)		 MK 875169.1 100.00
L1 大肠杆菌属
(Enterobacter sp.)		 MK 318778.1 99.36
GAN1 泛菌属
(Pantoea sp.)		 FJ 357809.7 98.73
Y8 芽孢杆菌属
(Bacillus subtilis subsp.)		 MN 744459.1 100.00
Z4 环状芽孢杆菌属
(Bacillus circulans strain)		 CP 026033.1 99.86

Tab.5

Orthogonal test results of optimizing microorganism degumming"

试验
号		 A B C 空列D 残胶
率/%		 断裂强度/
(cN·dtex-1)
1 1 1 1 1 47.76 6.33
2 1 2 2 2 44.69 1.82
3 1 3 3 3 42.90 3.13
4 2 1 2 3 36.86 8.19
5 2 2 3 1 42.52 4.28
6 2 3 1 2 41.28 3.08
7 3 1 3 2 40.22 2.22
8 3 2 1 3 46.64 2.56
9 3 3 2 1 38.97 1.72
残胶率 K1 45.12 41.61 45.23 43.08
K2 40.22 44.62 40.17 42.06
K3 41.94 41.05 41.88 42.13
R 4.90 3.57 5.05 1.02
断裂强度 K1 3.76 5.58 3.99 4.11
K2 5.18 2.89 3.91 2.37
K3 2.17 2.64 3.21 4.63
R' 3.01 2.94 0.78 2.25

Tab.6

Table of variance analysis"

指标 方差来源 平方和 自由度 均方和 F比 显著性
残胶率 A 37.08 2 18.54 19.04 **
B 22.12 2 11.06 11.36 *
C 39.75 2 19.88 20.41 **
D 1.95 2 0.97
断裂
强度		 A 13.60 2 6.80 12.31 *
B 15.94 2 7.97 14.43 *
C 1.10 2 0.55
D 8.41 2 4.20 7.61

Tab.7

Verification of deguming results"

菌株 残胶
率/%		 菌株 残胶
率/%
Z4 57.70 C6/1(Z4,Y8,GAN1,2-1,L1,X7) 50.74
YE13 57.00 C6/2(Z4,YE13,Y8,2-1,L1,X7) 44.06
Y8 55.50 C6/3(YE13,Y8,GAN1,2-1,L1,X7) 42.86
GAN1 50.90 C6/4(Z4,YE13,GAN1,2-1,L1,X7) 38.60
2-1 48.40 C6/5(Z4,YE13,Y8,GAN1,2-1,X7) 34.70
L1 47.70 C6/6(Z4,YE13,Y8,GAN1,2-1,L1) 32.19
X7 46.64 C6/7(Z4,YE13,Y8,GAN1,L1,X7) 31.04
C7(Z4,YE13,Y8,GAN1,2-1,L1,X7) 30.45
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