废弃羊毛角蛋白再生工艺及其高值化应用的研究进展

doi:10.13475/j.fzxb.20230203702

Abstract

Abstract:

Significance Excesive consumption of petroleum-based synthetic fibers limits the sustainability of textile industry. It is urgent to find low-cost renewable biomass resources to replace convenitional petroleum resources. Due to the quick expansion of fast fashion, the amount of waste wool grows every year. In addition, waste wool is normally disposed through incineration or landfill, which is not conducive to economic development and causes severe secondary pollution to the environment. Keratin is the major component of wool, which has excellent mechanical stability, shape memory properties, piezoelectric properties, biological activity, biodegradability, biocompatibility, and adsorption properties. Thus, keratinous material extracted from waste wool can be applied to produce high-value applications such as functional finishing agents, thermal and sound insulation materials, flexible electronic devices, biomedical applications, or adsorption materials. The regeneration technology of keratin effectively contributes to the sustainable development of wool resources, which triggered dramatically interest in recent years.

Progress This review mainly reports the regeneration techniques of keratin, which includes the methods of extraction and recent high-value applications in the form of fibers, membranes, gels, and scaffolds. The common methods of keratin extraction primarily include physical, chemical, and biological methods. Owing to precisely destroying disulfide bonds and hydrogen bonds, chemical methods, such as reduction, oxidation, ionic liquid, and deep eutectic solvent methods, are widely applied. The reduction methods effectively preserve the secondary structure of keratin, and the free sulfhydryl groups generated after extraction can be reconstructed in the subsequent process to cross-link keratin chains, thus perfectly restoring the hierarchical structure in fibers. However, keratinous materials are normally brittle due to poor mechanical properties. In previous work, researchers had demonstrated that 1,4-dithiothreitol can effectively modulate the viscoelastic spinning dope and act as a bridge in the keratin chains. After the drafting in wet spinning, fibers were induced to rearrange to the secondary structure. The regenerated keratin materials with excellent mechanical properties have extensive application prospects, which mainly include flexible electronic devices (such as humidity sensors, conductive composites, gel electrolytes, and so on), biomedical productions (such as wound healing, hemostasis, drug release carriers, tissue engineering, and so on) as well as adsorption applications.

Conclusion and Prospect The regenerated techniques of waste wool keratin and its high-value applications receive a lot of attention, promoting the transformation of biomass resources into the valuable materials. However, there are still some challenges that prevent its further practical and commercial production, such as poor mechanical properties, toxicity of chemical reagents, higher cost, and non-environmentally friendly disposal techniques. In-depth investigations on keratin extraction techniques not only can ensure high extraction rate, but also guarantee integral secondary structure of keratin chains. For the extraction techniques, ionic liquids, and green reducing agents such as cysteine, glutathione, and lower toxic 1,4-dithiothreitol gain widespread attention. In addition, chemical methods to enhance the extraction rate of keratin can be assisted by ultrasonic or microwave treatment. A dearth of information regarding the improvement of mechanical property has far limited the process of industrialization. It is also suggested that further investigations are required to gain high molecular weight keratin, find eco-friendly cross-linking agents, increase the orientation and crystallinity of the prepared fibers. In order to reduce purification cost in the biomedical applications, researches should use green and environmentally friendly reagents in the extraction process. The rapid development of keratin regeneration techniques can greatly promote the recycling of biomass resources, and sustainable economic development.

Key words: wool, keratin, regeneration, hierarchical structure, high-value application

CLC Number:

TS102.6

DONG Yalin, WANG Liming, QIN Xiaohong. Research progress in regeneration process and high-value applications of waste wool keratin[J].Journal of Textile Research, 2024, 45(07): 213-222.

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URL: http://www.fzxb.org.cn/EN/10.13475/j.fzxb.20230203702

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References 69

[1]	CUI G, DONG Y, LI B, et al. A novel heterogeneous Fenton photocatalyst prepared using waste wool fiber combined with Fe³⁺ ions for dye degradation[J]. Fibers and Polymers, 2017, 18: 713-719.
[2]	KYERE V N, GREVE K, ATIEMO S M, et al. Contamination and health risk assessment of exposure to heavy metals in soils from informal e-waste recycling site in Ghana[J]. Emerging Science Journal, 2018, 2(6): 428-436.
[3]	SUN Y, LI B, ZHANG Y, et al. The progress and prospect for sustainable development of waste wool resources[J]. Textile Research Journal, 2022, 93(1-2): 468-485.
[4]	SHAVANDI A, SILVA T H, BEKHIT A A, et al. Keratin: dissolution, extraction and biomedical application[J]. Biomater Sci, 2017, 5(9): 1699-1735.
[5]	SU C, GONG J S, QIN J, et al. Glutathione enables full utilization of wool wastes for keratin production and wastewater decolorization[J]. Journal of Cleaner Production, 2020. DOI: 10.1016/j.jclepro.2020.122092.
[6]	NEPAL D, KANG S, ADSTEDT K M, et al. Hierarchically structured bioinspired nano-composites[J]. Nat Mater, 2023, 22(1): 18-35.
[7]	CERA L, GONZALEZ G M, LIU Q, et al. A bioinspired and hierarchically structured shape-memory material[J]. Nat Mater, 2021, 20(2): 242-9.
[8]	QUARTINELLO F, VECCHIATO S, WEINBERGER S, et al. Highly selective enzymatic recovery of building blocks from wool-cotton-polyester textile waste blends[J]. Polymers, 2018. DOI: 10.3390/polym10101107.
[9]	金雨婷, 胡馨予, 石国庆, 等. 羊毛蛋白组学研究进展[J]. 中国细胞生物学学报, 2020, 42(7): 1276-1287.
	JIN Yuting, HU Xinyu, SHI Guoqing, et al. Advance in the research of wool proteomics[J]. Chinese Journal of Cell Biology, 2020, 42(7): 1276-1287.
[10]	CLARK M. Handbook of textile and industrial dyeing: principles, processes and types of dyes[M]. Cambridge: Woodhead Pubiling Liminted. Elsevier, 2011:51-53.
[11]	MCKITTRICK J, CHEN P Y, BODDE S, et al. The structure, functions, and mechanical properties of keratin[J]. JOM, 2012, 64(4): 449-468.
[12]	LAZARUS B S, CHADHA C, VELASCO-HOGAN A, et al. Engineering with keratin: a functional material and a source of bioinspiration[J]. iScience, 2021. DOI: 10.1016/j.isci.2021.102798.
[13]	王志诚, 王昊, 肖璇, 等. 角蛋白提取工艺及在环保材料领域应用研究现状分析[J]. 山东化工, 2022, 51(16): 82-3,8.
	WANG Zhicheng, WANG Hao, XIAO Xuan, et al. Analysis of keratin extraction technology and its application in the field of environmental protection materials[J]. Shandong Chemical Industry, 2022, 51(16): 82-3,8.
[14]	FEUGHELMAN M. A two-phase structure for keratin fibers[J]. Textile Research Journal, 1959, 29(3): 223-228.
[15]	CHILAKAMARRY C R, MAHMOOD S, SAFFE S, et al. Extraction and application of keratin from natural resources: a review[J]. 3 Biotech, 2021, 11(5): 1-12.
[16]	KREPLAK L, DOUCET J, DUMAS P, et al. New aspects of the α-helix to β-sheet transition in stretched hard α-keratin fibers[J]. Biophysical Journal, 2004, 87(1): 640-647.
[17]	WANG B, YANG W, MCKITTRICK J, et al. Keratin: Structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration[J]. Progress in Materials Science, 2016, 76: 229-318.
[18]	FRASER R D B, MACRAE T P. The mechanical properties of biological materials[M]. London: Society for Experimental Biology, 1980: 211-246.
[19]	朱锦, 马宁, 邵元龙. 再生羊毛角蛋白纤维的制备及力学增强设计[J]. 功能材料与器件学报, 2021, 27(5): 371-382.
	ZHU Jin, MA Ning, SHAO Yuanlong. Preparation and reinforcing design of regenerated wool keratin fiber[J]. Journal of Functional Materials and Devices, 2021, 27(5): 371-382.
[20]	XIAO X, HU J, GUI X, et al. Is biopolymer hair a multi-responsive smart material[J]. Polymer Chemistry, 2017, 8(1): 283-294.
[21]	MENEFEE E. Charge separation associated with dipole disordering in proteins[J]. Annals of the New York Academy of Sciences, 1974, 238(1): 53-67.
[22]	李子晗, 赵超, 王闻宇, 等. 蛋白质压电材料的研究进展[J]. 材料导报, 2022, 36(11): 205-212.
	LI Zihan, ZHAO Chao, WANG Wenyu, et al. Research progress of protein piezoelectric materials[J]. Materials Reports, 2022, 36(11): 205-212.
[23]	MORI H, HARA M. Transparent biocompatible wool keratin film prepared by mechanical compression of porous keratin hydrogel[J]. Materials Science and Engineering: C, 2018, 91: 19-25.
[24]	ZHANG H, WANG K, GAO T, et al. Controlled release of bFGF loaded into electrospun core-shell fibrous membranes for use in guided tissue regen-eration[J]. Biomedical Materials, 2020. DOI: 10.1088/1748-605X/ab7979.
[25]	YIFANG S, HUANG L, WANG X, et al. Intelligent drug delivery system based on silk fibroin/wool keratin[J]. Mathematical Problems in Engineering, 2022. DOI: 10.1155/2022/6748645.
[26]	HUMPHRIES J D, BYRON A, HUMPHRIES M J. Integrin ligands at a glance[J]. Journal of Cell Science, 2006, 119(19): 3901-3903.
[27]	RAJABI M, ALI A, MCCONNELL M, et al. Keratinous materials: structures and functions in biomedical applications[J]. Mater Sci Eng C Mater Biol Appl, 2020. DOI: 10.1016/j.msec.2019.110612.
[28]	XU H, YANG Y. Controlled de-cross-linking and disentanglement of feather keratin for fiber preparation via a novel process[J]. ACS Sustainable Chemistry & Engineering, 2014, 2(6): 1404-1410.
[29]	OKORO O V, JAFARI H, HOBBI P, et al. Enhanced keratin extraction from wool waste using a deep eutectic solvent[J]. Chemical Papers, 2022, 76(5): 2637-2648.
[30]	XIE H, LI S, ZHANG S. Ionic liquids as novel solvents for the dissolution and blending of wool keratin fibers[J]. Green Chemistry, 2005, 7(8): 606-608.
[31]	ZHAO W, YANG R, ZHANG Y, et al. Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion[J]. Green Chemistry, 2012, 14(12): 3352-3360.
[32]	ZHANG Y, ZHAO W, YANG R. Steam flash explosion assisted dissolution of keratin from feathers[J]. ACS Sustainable Chemistry & Engineering, 2015, 3(9): 2036-2042.
[33]	LI X, GUO Z, LI J, et al. Swelling and microwave-assisted hydrolysis of animal keratin in ionic liquids[J]. Journal of Molecular Liquids, 2021. DOI: 10.1016/j.molliq.2021.117306.
[34]	DU W, ZHANG L, ZHANG C, et al. Green and highly efficient wool keratin extraction by microwave induction method[J]. Frontiers in Materials, 2022. DOI: 10.3389/fmats.2021.789081.
[35]	XU H, MA Z, YANG Y. Dissolution and regeneration of wool via controlled disintegration and disentanglement of highly crosslinked keratin[J]. Journal of Materials Science, 2014, 49(21): 7513-7521.
[36]	KATOH K, TANABE T, YAMAUCHI K. Novel approach to fabricate keratin sponge scaffolds with controlled pore size and porosity[J]. Biomaterials, 2004, 25(18): 4255-4262. pmid: 15046915
[37]	MI X, LI W, XU H, et al. Transferring feather wastes to ductile keratin filaments towards a sustainable poultry industry[J]. Waste Management, 2020, 115: 65-73. doi: S0956-053X(20)30388-3 pmid: 32731135
[38]	MATTIELLO S, GUZZINI A, DEL GIUDICE A, et al. Physico-chemical characterization of keratin from wool and chicken feathers extracted using refined chemical methods[J]. Polymers, 2022. DOI: 10.3390/polym15010181.
[39]	THOMPSON E, O'DONNELL I. Studies on reduced wool I: the extent of reduotion of wool with inoreasing conoentrations of thiol, and the extraotion of proteins from reduoed and alkylated wool[J]. Australian Journal of Biological Sciences, 1962, 15(4): 757-768.
[40]	FEROZ S, MUHAMMAD N, DIAS G, et al. Extraction of keratin from sheep wool fibres using aqueous ionic liquids assisted probe sonication technology[J]. Journal of Molecular Liquids, 2022. DOI: 10.1016/j.molliq.2022.118595.
[41]	DEB-CHOUDHURY S, PLOWMAN J E, HARLAND D P. Isolation and analysis of keratins and keratin-associated proteins from hair and wool[J]. Methods Enzymol, 2016, 568: 279-301.
[42]	ZHANG Z, NIE Y, ZHANG Q, et al. Quantitative change in disulfide bonds and microstructure variation of regenerated wool keratin from various ionic liquids[J]. ACS Sustainable Chemistry & Engineering, 2017, 5(3): 2614-2622.
[43]	JI Y, CHEN J, LÜ J, et al. Extraction of keratin with ionic liquids from poultry feather[J]. Separation and Purification Technology, 2014, 132: 577-83.
[44]	SU C, GONG J S, YE J P, et al. Enzymatic extraction of bio-active and self-assembling wool keratin for biomedical applications[J]. Macromolecular Bioscience, 2020. DOI: 10.1002/mabi.202000073.
[45]	POOLE A J, CHURCH J S, HUSON M G. Environmentally sustainable fibers from regenerated protein[J]. Biomacromolecules, 2009, 10(1): 1-8. doi: 10.1021/bm8010648 pmid: 19035767
[46]	XU H, MA Z, YANG Y. Dissolution and regeneration of wool via controlled disintegration and disentanglement of highly crosslinked keratin[J]. Journal of Materials Science, 2014, 49: 7513-7521.
[47]	ZHU J, MA N, LI S, et al. Reinforced wool keratin fibers via dithiol chain re-bonding[J]. Advanced Functional Materials, 2023. DOI: 10.1002/adfm.202213644.
[48]	LI Y B, LIU H H, WANG X C, et al. Fabrication and performance of wool keratin-functionalized graphene oxide composite fibers[J]. Materials Today Sustainability, 2019. DOI: 10.1016/j.mtsust.2019.100006.
[49]	CARINGELLA R, BHAVSAR P, DALLA FONTANA G, et al. Fabrication and properties of keratoses/sericin blend films[J]. Polymer Bulletin, 2022, 79(4): 2189-2204.
[50]	FERNáNDEZ-D'ARLAS B. Tough and functional cross-linked bioplastics from sheep wool keratin[J]. Scientific Reports, 2019, 9(1): 1-12.
[51]	CATALDI P, CONDURACHE O, SPIRITO D, et al. Keratin-graphene nanocomposite: transformation of waste wool in electronic devices[J]. ACS Sustainable Chemistry & Engineering, 2019, 7(14): 12544-12551.
[52]	LI Y B, LIU H H, WANG X C, et al. Fabrication and performance of wool keratin-functionalized graphene oxide composite fibers[J]. Materials Today Sustainability, 2019. DOI: 10.1016/j.mtsust.2019.100006.
[53]	ZHANG L, HU F, ZHU S, et al. Meso-reconstruction of wool keratin 3D 'molecular springs' for tunable ultra-sensitive and highly recovery strain sensors[J]. Small, 2020. DOI: 10.1002/smll.202000128.
[54]	HUANG H, DONG Z, REN X, et al. High-strength hydrogels: Fabrication, reinforcement mechanisms, and applications[J]. Nano Research, 2023(16): 3475-3515.
[55]	汤燕伟, 于伟东. 羊毛溶液和角蛋白膜的实用制备技术与基本问题[J]. 膜科学与技术, 2007(2): 80-84.
	TANG Yanwei, YU Weidong. Practical preparation techniques and basic problems of wool solution and keratin membrane[J]. Menbrane Science and Technology, 2007(2): 80-84.
[56]	郑顺姬, 曹向禹, 隋智慧. 角蛋白膜的制备及应用研究进展[J]. 化工新型材料, 2021, 49(4): 251-256.
	ZHENG Shunji, CAO Xiangyu, SUI Zhihui. Research progress of preparation and application of keratin film[J]. New Chemical Materials, 2021, 49(4): 251-256.
[57]	LINNES M P, RATNER B D, GIACHELLI C M. A fibrinogen-based precision microporous scaffold for tissue engineering[J]. Biomaterials, 2007, 28(35): 5298-5306. pmid: 17765302
[58]	LEE C H, YUN Y J, CHO H, et al. Environment-friendly, durable, electro-conductive, and highly transparent heaters based on silver nanowire functionalized keratin nanofiber textiles[J]. Journal of Materials Chemistry C, 2018, 6(29): 7847-7854.
[59]	ZHANG W, LIU X, LIN Y, et al. Palladium nanoparticles/wool keratin-assisted carbon composite-modified flexible and disposable electrochemical solid-state pH sensor[J]. Chinese Physics B, 2022. DOI: 10.1088/1674-1056/ac3ca9.
[60]	PATIL A B, MENG Z, WU R, et al. Tailoring the meso-structure of gold nanoparticles in keratin-based activated carbon toward high-performance flexible sensor[J]. Nanomicro Lett, 2020. DOI: 10.1007/s40820-020-00459-5.
[61]	HAMMOUCHE H, ACHOUR H, MAKHLOUF S, et al. A comparative study of capacitive humidity sensor based on keratin film, keratin/graphene oxide, and keratin/carbon fibers[J]. Sensors and Actuators A: Physical, 2021. DOI: 10.1016/j.sna.2021.112805.
[62]	SHAO Y, ZHAO J, HU W, et al. Regulating interfacial ion migration via wool keratin mediated biogel electrolyte toward robust flexible Zn-ion batteries[J]. Small, 2022. DOI: 10.1002/smll.202107163.
[63]	FEROZ S, MUHAMMAD N, RATNAYAKE J, et al. Keratin-based materials for biomedical applic-ations[J]. Bioactive Materials, 2020, 5(3): 496-509.
[64]	BORDELEAU F, BESSARD J, SHENG Y, et al. Keratin contribution to cellular mechanical stress response at focal adhesions as assayed by laser tweezers[J]. Biochemistry and Cell Biology, 2008, 86(4): 352-359. doi: 10.1139/o08-076 pmid: 18756330
[65]	SIERPINSKI P, GARRETT J, MA J, et al. The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves[J]. Biomaterials, 2008, 29(1): 118-128. pmid: 17919720
[66]	SANCHEZ RAMIREZ D O, CRUZ-MAYA I, VINEIS C, et al. Design of asymmetric nanofibers-membranes based on polyvinyl alcohol and wool-keratin for wound healing applications[J]. J Funct Biomater, 2021. DOI: 10.3390/jfb12040076.
[67]	ZHU S, ZENG W, MENG Z, et al. Using wool keratin as a basic resist material to fabricate precise protein patterns[J]. Advanced Materials, 2019. DOI: 10.1002/adma.201900870.
[68]	TISSERA N D, WIJESENA R N, YASASRI H, et al. Fibrous keratin protein bio micro structure for efficient removal of hazardous dye waste from water: surface charge mediated interfaces for multiple adsorption desorption cycles[J]. Materials Chemistry and Physics, 2020. DOI: 10.1016/j.matchemphys.2020.122790.
[69]	ALUIGI A, CORBELLINI A, ROMBALDONI F, et al. Wool-derived keratin nanofiber membranes for dynamic adsorption of heavy-metal ions from aqueous solu-tions[J]. Textile Research Journal, 2013, 83(15): 1574-1586.

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样品	制备工艺	断裂强度/ MPa	断裂伸长率/%	文献
天然羊毛	—	173±23	36±5	[45]
再生角蛋白纤维	添加表面活性剂提高纺丝性能	101±15	10.9±2.9	[46]
再生角蛋白纤维	氧化重建交联	137	100	[7]
再生角蛋白纤维	引入DTT扩链剂	186.1	8	[47]
角蛋白/ PEG-g-GO 复合纤维	聚乙二醇功能化的氧化石墨(PEG-g-GO) 重建角蛋白	157±40	3.9	[48]

Research progress in regeneration process and high-value applications of waste wool keratin

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