Abstract: For enhancing the decolorizing effect of dye-house wastewater,a decoloring method using bacteria immobilized on sepiolite was developed.And decolorization of Acid Red B by immobilized cell was investigated.The effect of temperature,pH value,the amount of mycelium on the rate of discolorization was studied.The optimum decolorizing process conditions by bacteria immobilized on sepiolite were as follows: pretreating temperature 400℃,decolorizing time 72 h,33℃,pH 7.0,and the amount of mycelium 2%,and under these conditions 91.5% of decolorizing rate of Acid Red B was achieved.The experimental results show that when dye concentration is less than 100 mg/L,the discolorizing effect of bacteria immobilized on sepiolite is advantageous over that of free suspension bacteria.