Abstract: For the issue of interactive effect between warp and weft of fabric under practical working environment,the mechanical properties of ramie fiber fabric ，which is at 0 °、 15 °、30 °、45 °、60 °、75 °、 90 ° seven plane direction and set different load threshold values at warp direction ，were investigated by biaxial tensile test, and strain filed were characterized by using digital speckle correlation. The results showed that: interactive effect between warp and weft existed and the influence of load at weft to warp were larger than warp to weft，as the load at warp direction linear increased, force values at warp direction nonlinear increased and load ratio，the absolute value of strain at warp and weft direction decreased，but Poissons ratio kept the same essentially since the average and coefficient of variation respectively were: 0.338、0.484%. As the angle for X axis and warp direction increased，the loading mean value curve at x and Y axis had sine-curve characteristic，and there were axisymmetric distribution trend for loading box charts at 15°and75 °，30°and 60°with45°. The greatest interactive effect happened, when the warp-load is about 62.2%，65.3% of the unaxial and biaxial breaking strength, and the maximum rates of load and strain respectively were: 2.593 N/S，0.092/S.