Abstract: The adsorption and degradation of azo dyes by mycelium pellets7 were studied in open system, including the conditions such as the cultivation time of mycelium, the dosage of carbon and nitrogen, the dosage of mycelium , the time of adsorption and degradation and the reusing of mycelium. The results showed that the mycelium cultivated for 36h could grow, adsorb and degrade dyes in the conditions of glucose 2.5g/L, NaNO30.5 g/L, KH2PO4 1.0g/L, MgSO4 0.5g/L. The ultraviolet and experimental observations of inoculum of the dyes after dealt with mycelium pellets7 showed that the removing of acid purple by mycelium pellets7 was with degradation and the removing of reactive brilliant red dyes by mycelium pellets7 was with adsorption. In open system, the mycelium put up better growth vigor, ballability, resue and repellency for contaminating microorganism.