In order to better develop and utilize the resources of dog hair, the influences of wet / heat treatment on the structure and properties of dog hair were studied. The dog hair were subjected to heat and wet treatment, respectively, then the fibers were tested by single fiber universal tester, Fourier transform infrared spectroscopy, X-ray diffraction, thermal analyzer, thermal insulation tester and scanning electron microscopy. The results show that the crimp ratio of dog hair decreases after wet treatment, while that of the fiber increases after heat treatment. Heat treatment has much heavier harm to the surface of that fiber than wet treatment, and the mechanical properties of the dog hair are significantly worse after heat treatment. The dog hair has the infrared characteristics of protein fiber, and both of the wet treatment and heat treatment have no obvious influence on its molecular structure, but the secondary structure is affected. The crystallization indexes of samples after wet treatment and heat treatment decrease from 56.87% of untreated sample to 46.21% and 45.92%, respectively, and the heat stability decreases. In addition, the hollow ratio of the wet treated dog hair is decreased obviously and the warm retention ratio is reduced accordingly. However, the hollow ratio of the fibers after heat treatment increases, and the warm retention ratio increases to 96.88%.