In order to study the relationship of local thermal insulation between standing and walking states and the relationship of the total thermal insulation between the human testing and thermal manikin testing, the heat flow density of the 10 parts of human body were measured by the heat flow meter method. Firstly, the thermal insulation of standing and walking were calcuated by the area coefficient. The average value of the radio of four ensembles was calculated as the final radio of thermal insulation. Secondly, the dynamic and static thermal insulationof each part were linearly fitted. The relationship between the thermal resistance ratio and the slope of the fitted straight line was evaluated. The results show that the dynamic thermal resistance is significantly lower than the static thermal resistance, and the radio of dynamic and static follows by 0.72,0.75,0.87,0.74,0.75,0.83,0.82,0.95,0.61,0.64. The parts that the slope smaller than or equal to 0.76 is significantly affected by the moving. Finally, the digital model between static and dynamic thermal insulation of the human testing, the relationship between the human testing and thermal manikin testing under dynamic condition were obtained.