In order to develop natural wrinkle-resistant fabrics, the effect of spinning methods on fabric tensile elasticity and wrinkle-resistance was investigated. In this research, five types of 14.8 tex yarns were prepared using polyester (PET) filament and pure cotton yarn through complete condensing spinning, complete condensing spinning core-spun, complete condensing Siro-spinning core-spun, complete condensing Siro-spinning double-core core-spun and plied yarns. Five types of fabrics of the same specification were woven from these 5 types of yarns. Performances of the five fabrics were evaluated and analyzed for shape retention and deformation resistance, such as wrinkle resistance, dimensional stability, rigid flexibility and tensile deformation. The results show that the greater elastic deformation ratio and initial modulus of the fiber, the better the elasticity of the fabric, and the better the wrinkle recovery of the fabric. When the yarn is less in hairiness, smooth and even in surface, and high in initial modulus, the fabric demonstrates higher wrinkle resistance. The complete condensing Siro-spinning core-spun yarn and plied yarns show good wrinkle resistance, dimensional stability and tensile elastic recovery and crisp, and the apparent flatness grade of fabrics both is 3.5. Compared with the plied yarn, the production process of complete condensing Siro-spinning core-spun is short and the cost is low, and therefore it is more suitable for the preparation of high shape retention care-free shirt fabrics.