To solve the difficult thermoplastic processing and single function of polyvinyl alcohol(PVA), krill protein(AKP) and water were used as plasticizers to prepare PVA/AKP fibers by melt spinning. The hydrogen bonding in PVA/AKP fiber was characterized by infrared spectrometer, and the mechanism of hydrogen bonding was analyzed. Additionally, the crystallinity, morphology, thermal properties and mechanical properties of PVA/AKP fibers were characterized by X-ray diffractometer, scanning electron microscope, differential scanning calorimeter and single fiber strength tester, respectively. The results show that with the increase of AKP mass fraction, the content of intramolecular hydrogen bond and crystallinity of PVA/AKP fibers firstly increases and then decreases. When the mass fraction of AKP is 2%, the content of intramolecular hydrogen bond in PVA/AKP fibers reaches the maximum value (85.37%), and the crystallinity also reaches the maximum value (48%), and the fiber breakage strength reaches a maximum of 2.15 cN/dtex. AKP is helpful to improve the surface smoothness of the fiber, the fiber cross section presents regular round. Additionally, PVA/AKP fibers are more responsive to alkaline solutions, and the water absorption rate of the fiber is a constant of 36% after 2 h.