In order to solve the problem of property degradation of ramie fiber due to easy oxidation during degumming process using glycol organic solvent, anthraquinone (AQ) as degumming additive was used with glycol solvent. The properties of fibers prepared with different amounts of AQ were characterized, including polymerization degree, crystallinity, hemicellulose contents, physical and mechanical properties, and so on. The results show that the hemicellulose contents increase when AQ concentration arises. Meanwhile, the polymerization degree value, crystallinity, physical and mechanical properties of fiber show formation of peaks with the increase of AQ content. Under the best AQ mass fraction of 0.3%, the polymerization degree value, crystallinity, yield, tenacity, elongation and fracture work of fiber increase by 7.32%, 20.53%, 1.46%, 9.55%, 6.89%, 33.33%, respectively, while the residual gum content and density of fiber decrease by 17.91%, 7.24%, respectively.