In order to explore the structure and properties of polyester-based Ag/Cu films, nanoscale films were prepared by direct current and radio frequency co-sputtering using pure polyester fabrics of different structures as the substrate and metallic silver and copper as targets. Field emission scanning electron microscopy, X-ray diffractometry, photoelectron spectroscopy, fabric inductive electrostatic analysis, fabric UV protection and electromagnetic radiation test were utilized to characterize the surface morphology and structure and antistatic, anti-UV and anti-electromagnetic shielding performance of the samples. The experimental results show that the film on the surface of the nonwoven fabric is uniform and continuous, the diameter of the nano-particles on the surface of the woven fabric is small, and the surface distribution of the knitted fabric is continuous, but some island-like particles still exist. Cu(111), Cu(200), Ag(111) and Ag(200) diffraction peaks of Cu/Ag film on the surface of the sample are not obvious, and the crystallinity is low. XPS image shows that the nano-film is not oxidized, and exists in elemental form. The nonwoven fabric is used as the substrate, the antistatic, anti-UV, anti-electromagnetic shielding performance of the sample is the best, and the knitted fabric is the worst.